SARS-CoV-2 RNA-dependent RNA polymerase as a target for high-throughput drug screening

作者：Jiahui Wu, Zhiqiang Chen, Xue Han, Qiaoqiao Chen, Yintao Wang, Tingting Feng · 发表于：Future Virology · 年份：2023 · DOI：10.2217/fvl-2021-0335 · 被引用次数：12 · 研究领域：SARS-CoV-2 and COVID-19 Research、Viral gastroenteritis research and epidemiology、interferon and immune responses

The ongoing COVID-19 pandemic caused by the SARS-CoV-2 has necessitated rapid development of drug screening tools. RNA-dependent RNA polymerase (RdRp) is a promising target due to its essential functions in replication and transcription of viral genome. To date, through minimal RNA synthesizing machinery established from cryo-electron microscopy structural data, there has been development of high-throughput screening assays for directly screening inhibitors that target the SARS-CoV-2 RdRp. Here, we analyze and present verified techniques that could be used to discover potential anti-RdRp agents or repurposing of approved drugs to target the SARS-CoV-2 RdRp. In addition, we highlight the characteristics and application value of cell-free or cell-based assays in drug discovery.