Polydopamine-coated 3D-printed β-tricalcium phosphate scaffolds to promote the adhesion and osteogenesis of BMSCs for bone-defect repair: mRNA transcriptomic sequencing analysis

作者：Xin Sun, Xin Jiao, Zengguang Wang, Jie Ma, Tianchang Wang, Dan Zhu, Han Li, Liang Tang, Heyue Li, Changde Wang, Yiming Li, Xu Chen, Jinwu Wang, Yaogai Gan, Wenjie Jin · 发表于：Journal of Materials Chemistry B · 年份：2023 · DOI：10.1039/d2tb02280j · 被引用次数：26 · 研究领域：Polymer Surface Interaction Studies、Bone Tissue Engineering Materials、Electrospun Nanofibers in Biomedical Applications

Moreover, the expression of osteogenesis-related genes, such as R-spondin 1 and chemokine c-c-motif ligand 2, was increased. Finally, both the 3D-printed β-TCP scaffolds and PDA-coated scaffolds could significantly accelerate the formation of new bone in critical-size calvarial defects in rats compared with the control group; and the new bone formation was obviously higher in the PDA-coated scaffolds than in β-TCP scaffolds. In summary, 3D-printed β-TCP scaffolds with a PDA coating can improve the physicochemical characteristics and cellular bioactivity of the scaffold surface for bone regeneration. Potential differential genes were identified, which can be used as a foundation for further research.