Preparation of Silk Fibroin/Chitosan/Nano-Hydroxyapatite and Its Effect on the Repair of Cartilage Damage in Rats

作者：Dongfang Zhao, Shuqin Xing, Jiao Qi, Zhiqiang Wei, Jianghai Huang, Jigao Sun, Xinzhu Wen, Yafei Wang · 发表于：Science of Advanced Materials · 年份：2022 · DOI：10.1166/sam.2022.4352 · 被引用次数：3 · 研究领域：Silk-based biomaterials and applications、Periodontal Regeneration and Treatments、Bone Tissue Engineering Materials

This study was aimed to prepare a silk fibroin (SF)/chitosan (CS)/nano-Hydroxyapatite (n-HAp) composite scaffold for repair of cartilage damage. A four-layer bionic scaffold SF/CS/n-HAp was constructed by cryogenic freezing and forming. Sodium Tripolyphosphate (STPP) was used as a cross-linking agent to post-process the scaffold to improve the mechanical strength of the scaffold. The various materials prepared were characterized. The SF/CS/n-HAp scaffold prepared in this study was cream-colored cylindrical and elastic. It can show variability after external force was applied, and can be restored to its original appearance after elimination of the external force. The diameter of the scaffold was 5 cm, and the total thickness was about 1 cm. The scanning electron microscope (SEM) results showed that the surface of the scaffold material was smooth, the pore size changed gradually, and the connectivity among the holes was good. The pore size distribution of the entire scaffold material was between 100 μ m∼300 μ m, and the pore size range was suitable for the adhesion, expansion, and migration of chondrocytes and osteoblasts. The mechanical performance test results showed that the composite scaffold had a compression modulus of 706 kPa, which can meet the mechanical performance requirements for repair of smaller articular cartilage damage. In addition, the porosity of each layer of the scaffold was more than 91%, which was conducive to the adhesion of cells on the surface of the m...