Data mining combines bioinformatics discover immunoinfiltration-related gene SERPINE1 as a biomarker for diagnosis and prognosis of stomach adenocarcinoma

作者：Yiyan Zhai, Xinkui Liu, Zhihong Huang, Jingyuan Zhang, Antony Stalin, Yingying Tan, Fanqin Zhang, Meiling Chen, Rui Shi, Jiaqi Huang, Chao Wu, Zhishan Wu, Shan Lu, Leiming You, Jiarui Wu · 发表于：Scientific Reports · 年份：2023 · DOI：10.1038/s41598-023-28234-7 · 被引用次数：30 · 研究领域：Ferroptosis and cancer prognosis、Cancer-related molecular mechanisms research、Cancer Immunotherapy and Biomarkers

Abstract Stomach adenocarcinoma (STAD) is a type of cancer which often at itsadvanced stage apon diagnosis and mortality in clinical practice. Several factors influencethe prognosis of STAD, including the expression and regulation of immune cells in the tumor microenvironment. We here investigated the biomarkers related to the diagnosis and prognosis of gastric cancer, hoping to provide insights for the diagnosis and treatment of gastric cancer in the future. STAD and normal patient RNA sequencing data sets were accessed from the cancer genome atlas (TCGA database). Differential genes were determined and obtained by using the R package DESeq2. The stromal, immune, and ESTIMATE scores are calculated by the ESTIMATE algorithm, followed by the modular genes screening using the R package WGCNA. Subsequently, the intersection between the modular gene and the differential gene was taken and the STRING database was used for PPI network module analysis. The R packages clusterProfiler, enrichplot, and ggplot2 were used for GO and KEGG enrichment analysis. Cox regression analysis was used to screen survival-related genes, and finally, the R package Venn Diagram was used to take the intersection and obtain 7 hub genes. The time-dependent ROC curve and Kaplan–Meier survival curve were used to find the SERPINE1 gene, which plays a critical role in prognosis. Finally, the expression pattern, clinical characteristics, and regulatory mechanism of SERPINE1 were analyzed in STAD. We revealed t...