Translational patterns of ionotropic glutamate and GABA receptors during brain development and behavioral stimuli revealed by polysome profiling

作者：Yinghui Peng, Miaoqi Huang, Jiaoyan Xie, Beiyi Shu, Xiaojun Wang, Yumei Liao, Junyu Xu, Lei Shi · 发表于：Journal of Neurochemistry · 年份：2023 · DOI：10.1111/jnc.15771 · 被引用次数：5 · 研究领域：Neuroscience and Neuropharmacology Research、RNA Research and Splicing、Biochemical effects in animals

Abstract mRNA translation is critical for regulation of various aspects of the nervous system. Ionotropic glutamate and gamma‐aminobutyric acid type A (GABA A ) receptors are fundamental synaptic ion channels that control excitatory and inhibitory synaptic transmission, respectively. However, little is known about the translation of these receptors during brain development and function. By utilizing polysome profiling, a powerful tool for investigating translational machinery and mRNA translational states, we characterized the translational patterns of α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid (AMPA), N‐methyl‐ d ‐aspartate (NMDA), and GABA A receptor subunits, and compared them with total mRNA and protein levels during mouse brain development, in different brain regions, and in response to behavioral stimuli. Most of the receptor subunits exhibited developmental changes at total mRNA, translation, and protein levels, among which translation of Gria1 , Gria2 , Grin1 , Grin2a , Gabra1 , and Gabrg2 contributed greatly to their protein levels. Most of the receptor subunits also displayed differentiated levels of total mRNA, translation, and protein in the prefrontal cortex and hippocampus, among which translation of Gria1 , Gria2 , Gabrb2 , and Gabrg2 contributed to their protein levels. Finally, we showed that acute foot shock stress had a rapid influence in both the prefrontal cortex and hippocampus, with the prefrontal cortex displaying more changes at translationa...