Dynamic changes of the proportion of HLA-DR and CD38 coexpression subsets on T lymphocytes during IFN-based chronic hepatitis B treatment

作者：Yanjie Lin, Ge Shen, Si Xie, Xiaoyue Bi, Huihui Lu, Yang Liu, Tingting Jiang, Wen Deng, Shiyu Wang, Lu Zhang, Yao Lu, Yuanjiao Gao, Hongxiao Hao, Shuling Wu, Ruyu Liu, Min Chang, Mengjiao Xu, Leiping Hu, Xiaoxue Chen, Ronghai Huang, Minghui Li, Yao Xie · 发表于：Frontiers in Immunology · 年份：2023 · DOI：10.3389/fimmu.2022.1116160 · 被引用次数：9 · 研究领域：Hepatitis B Virus Studies、Hepatitis C virus research、Hepatitis Viruses Studies and Epidemiology

Background To investigate the changes of human leukocyte antigen DR (HLA-DR) and CD38 coexpression subsets on T lymphocytes following interferon (IFN) therapy for those who have chronic hepatitis B (CHB). Methods A prospective cohort of CHB patients participated in this study. CHB patients without IFN treatment (including naïve and nucleoside [nucleotide] analogs [NAs]-treated patients) were given pegylated interferon alfa (Peg-IFNα) treatment. Peripheral blood samples were taken at baseline, 4 weeks and 12-24 weeks of Peg-IFNα treatment. For the patients who entered the Peg-IFNα plateau phase due to the stagnation of the decrease in HBsAg, and Peg-IFNα was discontinued and Peg-IFNα therapy was resumed after an interval of 12-24 weeks. During the interval, they received first-line NAs treatment. Peripheral blood samples were collected at the baseline of the plateau phase, 12-24 weeks of intermittent treatment, and 12-24 weeks of Peg-IFNα retreatment. The peripheral blood samples were taken to determine virological, serological and biochemical indices of hepatitis B virus (HBV), and T lymphocyte related phenotypes were detected using flow cytometry. Results In the process of long-term treatment of Peg-IFNα, the percentage of HLA-DR + CD38 dim subsets increased significantly at first, then decreased gradually, while the percentage of HLA-DR + CD38 hi subsets markedly increased. During long-term Peg-IFNα treatment, there was a considerable negative correlation between HBsAg and ...