The impact of osmotic stresses on the biofilm formation, immunodetection, and morphology of Aeromonas hydrophila

作者：Wenbin Wang, Wenbin Wang, Ye Cao, Shuaichen Lu, Shuaichen Lu, Hongxing Ge, Saikun Pan, Saikun Pan, Xiaoyi Pan, Luxin Wang · 发表于：Microbiological Research · 年份：2023 · DOI：10.1016/j.micres.2023.127301 · 被引用次数：13 · 研究领域：Aquaculture disease management and microbiota、Bacterial biofilms and quorum sensing、Vibrio bacteria research studies

Aeromonas hydrophila (Ah) is a zoonotic pathogen of great importance to aquaculture and human health. This study systematically evaluated the impact of salinity, sugar, ammonia nitrogen, and nitric nitrogen levels on the fitness of Ah by using Luria-Bertani (LB) broth supplemented with different concentrations of NaCl, sucrose, NH 4 Cl, urea, NaNO 2 or NaNO 3 . Results showed that the static biofilm formation of Ah was higher at 28 °C compared to 37 °C ( P < 0.05). At 28 °C, as the NaCl (>1 %) and sucrose levels increased, the Ah biofilm formation and the binding between Ah cells and monoclonal antibodies (mAbs, for immunodetection) decreased. Elevated ammonia nitrogen and nitric nitrogen levels generated no significant impact on Ah biofilm formation or immunodetection ( P > 0.05). The expression of mAbs-targeted Omp remained unchanged under high NaCl or sucrose conditions. Further analysis showed that high sucrose conditions led to the over-expression of the extracellular polysaccharides (PS) and promoted the formation of capsule-like structures. These over-expressed PS and capsule structures might be one reason explaining the inhibited immunodetection efficacy. Results generated from this study provide crucial insights for the design of recovery and detection protocols for Ah present in food or environmental samples.