m7G-related gene NUDT4 as a novel biomarker promoting cancer cell proliferation in lung adenocarcinoma

作者：Yafei Liu, Bin Jiang, Chunjie Lin, Wanyinhui Zhu, Dingrui Chen, Yinuo Sheng, Zhiling Lou, Zhiheng Ji, Chuanqiang Wu, Ming‐Tsang Wu · 发表于：Frontiers in Oncology · 年份：2023 · DOI：10.3389/fonc.2022.1055605 · 被引用次数：18 · 研究领域：RNA modifications and cancer、Metalloenzymes and iron-sulfur proteins、Ubiquitin and proteasome pathways

Background: Lung cancer is the leading cause of mortality in cancer patients. N7-methylguanosine (m7G) modification as a translational regulation pattern has been reported to participate in multiple types of cancer progression, but little is known in lung cancer. This study attempts to explore the role of m7G-related proteins in genetic and epigenetic variations in lung adenocarcinoma, and its relationship with clinical prognosis, immune infiltration, and immunotherapy. Methods: Sequencing data were obtained from the Genomic Data Commons (GDC) Data Portal and Gene Expression Omnibus (GEO) databases. Consensus clustering was utilized to distinguish m7G clusters, and responses to immunotherapy were also evaluated. Moreover, univariate and multivariate Cox and Least absolute shrinkage and selection operator LASSO Cox regression analyses were used to screen independent prognostic factors and generated risk scores for constructing a survival prediction model. Multiple cell types such as epithelial cells and immune cells were identified to verify the bulk RNA results. Short hairpin RNA (shRNA) Tet-on plasmids, Clustered Regularly Interspaced Short Palindromic Repeats CRISPR/Cas9 for knockout plasmids, and nucleoside diphosphate linked to moiety X-type motif 4 (NUDT4) overexpression plasmids were constructed to inhibit or promote tumor cell NUDT4 expression, then RT-qPCR, Cell Counting Kit-8 CCK8 proliferation assay, and Transwell assay were used to observe tumor cell biological fun...