Susceptibility of kuruma shrimp to the infection with Decapod iridescent virus 1

作者：Liang Qiu, Xiao‐Meng Guo, Yong-Hui Feng, Jing‐Yi Xing, Xianyun Ren, Jie Huang · 发表于：Frontiers in Marine Science · 年份：2023 · DOI：10.3389/fmars.2023.1114123 · 被引用次数：7 · 研究领域：Invertebrate Immune Response Mechanisms、Aquaculture disease management and microbiota、Crustacean biology and ecology

Infection with Decapod iridescent virus 1 (iDIV1), an important emerging disease of shrimps and crabs, has been included in the Quarterly Aquatic Animal Disease Report (QAAD) by the Network of Aquaculture Centres in Asia-Pacific (NACA) and listed by the World Organization for Animal Health (WOAH). China has classified iDIV1 as a Class II animal pandemic disease. In the present study, to determine the susceptibility of Penaeus japonicus to Decapod iridescent virus 1 (DIV1), healthy kuruma shrimp were artificially infected with DIV1 (isolate SHIV 20141215) by per os (the pathway that mimics natural transmission) and intramuscular injection (invasive pathway). The infected P. japonicus showed clinical signs such as anorexia, retardation, evident reddish body, swollen and whitish lymphoid organs, and mortalities of almost 100%. Real-time PCR showed that all the challenged individuals by per os or intramuscular routes were DIV1-positive with an average virus load between 10 (9.09 ± 0.58) and 10 (8.94 ± 0.45) copies/μg-DNA, respectively. Histological examination revealed karyopyknosis, and eosinophilic inclusions and minute basophilic stains were combined in lymphoid organs, hematopoietic tissue and gills of diseased individuals. In addition, lymphoid organs showed disorganization of the tubule matrix. In situ DIG-labeling loop-mediated isothermal amplification (ISDL) also demonstrated the presence of DIV1 signals existed in lymphoid organs, hemopoietic tissue, gills, epithelial ti...