miR-150-5p in neutrophil-derived extracellular vesicles associated with sepsis-induced cardiomyopathy in septic patients

作者：Rongzong Ye, Qiuyun Lin, Wenkai Xiao, Lixia Mao, Pengfei Zhang, Lingshan Zhou, Xiaoxia Wu, Nannan Jiang, Xi-He Zhang, Yinhua Zhang, Daqing Ma, Jiahao Huang, Xiaoyan Wang, Liehua Deng · 发表于：Cell Death Discovery · 年份：2023 · DOI：10.1038/s41420-023-01328-x · 被引用次数：30 · 研究领域：Neutrophil, Myeloperoxidase and Oxidative Mechanisms、Extracellular vesicles in disease、Immune cells in cancer

INTRODUCTION: Early diagnosis and potential therapeutic targets of sepsis-induced cardiomyopathy (SIC) remain challenges clinically. Circulating extracellular vesicles from immune cells carrying crucial injurious mediators, including miRNAs in sepsis. However, the impacts of neutrophil-derived extracellular vesicles and their miRNAs in the SIC development are unknown. OBJECTIVES: The present study focused on the in-depth miRNA expression profiles of neutrophil-derived extracellular vesicles and explored the potential molecular biomarkers during the process of SIC. METHODS: Neutrophil-derived extracellular vesicles were isolated from the blood samples in three sepsis patients with or without cardiomyopathy on day 1 and day 3 after ICU admission in comparison with three healthy controls. miRNAs were determined by RNA sequencing. The closely related differentially expressed miRNAs with SIC were further validated through qRT-PCR in the other cohorts of sepsis patients with (30 patients) or without cardiomyopathy (20 patients) and the association between miRNAs and the occurrence or disease severity of septic cardiomyopathy were stratified with logistic regression analysis. RESULTS: Sixty-eight miRNAs from neutrophil-derived extracellular vesicles were changed significantly between healthy controls and without septic cardiomyopathy patients (61 miRNAs upregulated and seven downregulated). Thirty-eight miRNAs were differentially expressed in the septic cardiomyopathy patients. 27 c...