[Hydroxysafflor yellow A attenuates heat stroke-induced acute lung injury in mice by inhibiting necroptosis].

作者：X. Liu, Sian Zhang, Runjie Yu, Xue Lin, Wenhao Fan, Y Wang, Zhuowen Liang, W Xie, Yicheng Liu, Hongtian Chen · 发表于：PubMed · 年份：2022 · DOI：10.12122/j.issn.1673-4254.2022.12.17 · 被引用次数：1 · 研究领域：Pharmacology、Medicine、Andrology、Chemistry、Pathology、Immunology、Internal medicine、Biochemistry

OBJECTIVE: To investigate the protective effect of hydroxysafflor yellow A (HSYA) against heat stroke (HS)-induced acute lung injury and its possible mechanism. METHODS: intraperitoneal injection prior to HS was determined in a mice by observing heat tolerance of the mice. C57BL/6J mice were pretreated with HSYA at the optimal dose or with Nec-1 (a RIP1 activation inhibitor) before HS, and the changes in core body temperature and survival of the mice were observed during the 72-h recovery period. At different stages of recovery, lung tissues, bronchoalveolar lavage fluid and blood samples were collected from the mice for assessing lung tissue pathology, wet-to-dry weight ratio and water content of the lungs; leukocyte and neutrophil counts, total protein levels and HMGB1 level in the bronchoalveolar lavage fluid (BLF) were also detected. Serum levels of TNF-α, IL-6 and HMGB1 were detected with ELISA, and the expression levels of RIP1, RIP3, MLKL-s358, MLKL and MLKL-s358 proteins in the lung tissues were detected using Western blotting. RESULTS: <0.05), which were obviously lowered by HSYA pretreatment of the mice. CONCLUSION: Severe HS results in necroptosis in the lung tissue of mice, which can be alleviated by HSYA pretreatment.