Red-fleshed apple flavonoid extract alleviates CCl4-induced liver injury in mice

作者：Yizhou Chen, Yanbo Wang, Shenghui Jiang, Jihua Xu, Bin Wang, Xiaohong Sun, Yugang Zhang · 发表于：Frontiers in Nutrition · 年份：2023 · DOI：10.3389/fnut.2022.1098954 · 被引用次数：11 · 研究领域：Drug-Induced Hepatotoxicity and Protection、Pharmacological Effects of Natural Compounds、Liver Disease Diagnosis and Treatment

In recent years, the global incidence of liver damage has increased. Despite the many known health benefits of red-fleshed apple flavonoids, their potential liver-protective effects have not yet been investigated. In this study, we analyzed the composition of red-fleshed apple flavonoid extract (RAFE) by high-performance liquid chromatography (HPLC). We then induced liver damage in mice with carbon tetrachloride (CCl 4 ) and performed interventions with RAFE to analyze its effect on liver damage, using bifendate as a positive control. The results showed that catechin was the most abundant flavonoid in ‘XJ4’ RAFE (49.346 mg/100 g). In liver-injured mice, the liver coefficients converged to normal levels following RAFE intervention. Moreover, RAFE significantly reduced the enzymatic activity levels of glutamic oxaloacetic transaminase (ALT), glutamic alanine transaminase (AST), and alkaline phosphatase (ALP) in mouse serum. Furthermore, RAFE significantly increased the content or enzyme activity level of total glutathione, total antioxidant capacity, and superoxide dismutase, and significantly decreased the content of malondialdehyde in the liver of mice. In parallel, we performed histopathological observations of mouse livers for each group. The results showed that RAFE restored the pathological changes caused by CCl 4 around the central hepatic vein in mice and resulted in tightly bound hepatocytes. The recovery effect of RAFE was dose-dependent in the liver tissue. Regarding...