Establishment of a PPP2CA homozygous knockout human embryonic stem cell line via CRISPR/Cas9 system

作者：Tongbin Ding, Zhe Gao, Hang Liu, Yuan Li, Shichao Liu, Liguo Jian · 发表于：Stem Cell Research · 年份：2023 · DOI：10.1016/j.scr.2023.103029 · 研究领域：CRISPR and Genetic Engineering、Pluripotent Stem Cells Research、Advanced biosensing and bioanalysis techniques

Protein phosphatase 2A (PPP2CA) is one of the four main Ser/Thr phosphatase enzymes, which involved in the negative control of cell growth and division. PPP2CA is the main protein phosphatase in the heart, which regulates Ca (2+) through a series of ion channels and transporters. In this study, we generated a PPP2CA homozygous knockout human embryonic stem cell line WAe009-A-25 based on the transient expression CRISPR/Cas9 system to investigate functional effect of PP1 deficiency. This cell line has multidirectional differentiation potential, normal karyotypic and trilineage differentiation potential in vivo.