A multiepitope vaccine candidate against infectious bursal disease virus using immunoinformatics-based reverse vaccinology approach

作者：Irfan Gul, Amreena Hassan, Jan Mohd Muneeb, Towseef Akram, Ehtishamul Haq, Riaz Ahmad Shah, Nazir Ahmad Ganai, Syed Mudasir Ahmad, Naveed Anjum Chikan, Nadeem Shabir · 发表于：Frontiers in Veterinary Science · 年份：2023 · DOI：10.3389/fvets.2022.1116400 · 被引用次数：19 · 研究领域：vaccines and immunoinformatics approaches、Virology and Viral Diseases、Monoclonal and Polyclonal Antibodies Research

Infectious bursal disease virus is the causative agent of infectious bursal disease (Gumboro disease), a highly contagious immunosuppressive disease of chicken with a substantial economic impact on small- and large-scale poultry industries worldwide. Currently, live attenuated vaccines are widely used to control the disease in chickens despite their issues with safety (immunosuppression and bursal atrophy) and efficiency (breaking through the maternally-derived antibody titer). To overcome the drawbacks, the current study has, for the first time, attempted to construct a computational model of a multiepitope based vaccine candidate against infectious bursal disease virus, which has the potential to overcome the safety and protection issues found in the existing live-attenuated vaccines. The current study used a reverse vaccinology based immunoinformatics approach to construct the vaccine candidate using major and minor capsid proteins of the virus, VP2 and VP3, respectively. The vaccine construct was composed of four CD8 + epitopes, seven CD4 + T-cell epitopes, 11 B-cell epitopes and a Cholera Toxin B adjuvant, connected using appropriate flexible peptide linkers. The vaccine construct was evaluated as antigenic with VaxiJen Score of 0.6781, immunogenic with IEDB score of 2.89887 and non-allergenic. The 55.64 kDa construct was further evaluated for its physicochemical characteristics, which revealed that it was stable with an instability index of 16.24, basic with theoretical...