Remodeling of H3K9me3 during the pluripotent to totipotent-like state transition

作者：Hu Li, Jiatong Sun, Yu Dong, Yixin Huang, Li Wu, Chenxiang Xi, Zhongqu Su, Yihan Xiao, Chuyu Zhang, Yuwei Liang, Yujun Li, Zhiyi Lin, Lu Shen, Yixing Zuo, Abuduwaili Abudureheman, Jiqing Yin, Hong Wang, Xiangyin Kong, Rongrong Le, Shaorong Gao, Yanping Zhang · 发表于：Stem Cell Reports · 年份：2023 · DOI：10.1016/j.stemcr.2022.12.006 · 被引用次数：7 · 研究领域：Genomics and Chromatin Dynamics、CRISPR and Genetic Engineering、Pluripotent Stem Cells Research

Multiple chromatin modifiers associated with H3K9me3 play important roles in the transition from embryonic stem cells to 2-cell (2C)-like cells. However, it remains elusive how H3K9me3 is remodeled and its association with totipotency. Here, we integrated transcriptome and H3K9me3 profiles to conduct a detailed comparison of 2C embryos and 2C-like cells. Globally, H3K9me3 is highly preserved and H3K9me3 dynamics within the gene locus is not associated with gene expression change during 2C-like transition. Promoter-deposited H3K9me3 plays non-repressive roles in the activation of genes during 2C-like transition. In contrast, transposable elements, residing in the nearby regions of up-regulated genes, undergo extensive elimination of H3K9me3 and are tended to be induced in 2C-like transitions. Furthermore, a large fraction of trophoblast stem cell-specific enhancers undergo loss of H3K9me3 exclusively in MERVL + / Zscan4 + cells. Our study therefore reveals the unique H3K9me3 profiles of 2C-like cells, facilitating the further exploration of totipotency.