Phosphate, calcium, and vitamin D signaling, transport, and metabolism in the endometria of cyclic ewes

作者：Claire Stenhouse, Makenzie G Newton, Katherine M Halloran, Robyn M Moses, Nirvay Sah, Larry J. Suva, Fuller W. Bazer · 发表于：Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology · 年份：2023 · DOI：10.1186/s40104-022-00803-2 · 被引用次数：14 · 研究领域：Reproductive Physiology in Livestock、Reproductive Biology and Fertility、Reproductive System and Pregnancy

Abstract Background Recent evidence suggests important roles for progesterone (P4) and interferon tau in the regulation of calcium, phosphate, and vitamin D signaling in the uteri of pregnant sheep. However, the effects of P4 and estradiol (E2), with respect to the expression of their receptors PGR and ESR1, respectively, in uterine epithelia on mineral signaling during the estrous cycle has not been investigated. Estrous cycles of mature Suffolk ewes were synchronized, prostaglandin F2α was administered, and ewes were observed for estrus (designated as Day 0) in the presence of vasectomized rams. On Days 1, 9, or 14 of the estrous cycle, hysterectomies were performed. Results 25-hydroxyvitamin D was more abundant in plasma from ewes on Day 14 than Day 1 ( P < 0.05). Expression of fibroblast growth factor receptor 2 ( FGFR2) , a disintegrin and metalloprotease 17 ( ADAM17 ), and parathyroid hormone-related protein ( PTHrP) mRNAs was greater in endometria on Day 9 compared to Days 1 and 14 ( P < 0.01). Similarly, expression of transient receptor potential cation channel subfamily V member 6 ( TRPV6 ) mRNA was greater in endometria on Day 9 than Day 1 ( P < 0.05). ATPase plasma membrane Ca 2 + transporting 4 ( ATP2B4 ) and S100 calcium binding protein G ( S100G) mRNA expression was greater in endometria on Day 14 than on Days 1 and 9 ( P < 0.01). In contrast, endometrial expression of vitamin D receptor ( VDR ) mRNA was lower on Days 9 and 14 than Day 1 ( P < 0.0...