Plasma protein profiling analysis in patients with atrial fibrillation before and after three different ablation techniques

作者：Menglu Lin, Yangyang Bao, Zunhui Du, Yanting Zhou, Ning Zhang, Changjian Lin, Yinyin Xie, Yinyin Xie, Ruihong Zhang, Qiheng Li, Jin Wei Quan, Tingfang Zhu, Yuan Xie, Yuan Xie, Cathy Xu, Yun Xie, Yun Xie, Yue Wei, Qingzhi Luo, Wenqi Pan, Lingjie Wang, Tianyou Ling, Qi Jin, Liqun Wu, Tong Yin, Yucai Xie, Yucai Xie · 发表于：Frontiers in Cardiovascular Medicine · 年份：2023 · DOI：10.3389/fcvm.2022.1077992 · 被引用次数：4 · 研究领域：Atrial Fibrillation Management and Outcomes、Blood properties and coagulation、Cardiac Fibrosis and Remodeling

Background: There are controversies on the pathophysiological alteration in patients with atrial fibrillation (AF) undergoing pulmonary vein isolation using different energy sources. Objectives: We evaluated the changes in plasma proteins in acute phase post-ablation in patients receiving cryoballoon ablation, radiofrequency balloon ablation, or radiofrequency ablation. Methods: Blood samples from eight healthy controls and 24 patients with AF were taken on the day of admission, day 1, and day 2 post-ablation and analyzed by the Olink proximity extension assay. Proteins were identified and performed with enrichment analysis. Protein-protein interaction network and module analysis were conducted using Cytoscape software. Results: Of 181 proteins, 42 proteins in the cryoballoon group, 46 proteins in the radiofrequency balloon group, and 43 proteins in the radiofrequency group significantly changed after ablation. Most of the proteins altered significantly on the first day after ablation. Altered proteins were mainly involved in cytokine-cytokine receptor interaction. Both balloon-based ablations showed a similar shift toward enhancing cell communication and regulation of signaling while inhibiting neutrophil chemotaxis. However, radiofrequency ablation presented a different trend. Seed proteins, including osteopontin, interleukin-6, interleukin-10, C-C motif ligand 8, and matrix metalloproteinase-1, were identified. More significant proteins associated with hemorrhage and coagu...