Accurate, Sensitive, and Rapid Detection of Pseudomonas aeruginosa Based on CRISPR/Cas12b with One Fluid-Handling Step

作者：Xiaotong Qiu, Xueping Liu, Ruixue Wang, Xiao Ma, Lichao Han, Jiang Yao, Zhenjun Li · 发表于：Microbiology Spectrum · 年份：2023 · DOI：10.1128/spectrum.03523-22 · 被引用次数：16 · 研究领域：CRISPR and Genetic Engineering、Biosensors and Analytical Detection、Bacterial biofilms and quorum sensing

This study reports a P. aeruginosa CRISPR-top assay which can precisely identify P. aeruginosa using nucleic acids from pure cultures or clinical samples in one pot with one fluid-handling step. The P. aeruginosa CRISPR-top reaction is suitable for on-site testing, and its diagnostic performance can be compared with that of qPCR.