GhPRE1A promotes cotton fibre elongation by activating the DNA ‐binding bHLH factor GhPAS1

作者：Huanhuan Wu, Liqiang Fan, Mengzhen Guo, Le Liu, Lisen Liu, Liyong Hou, Lei Zheng, Ghulam Qanmber, Lili Lu, Jie Zhang, Fuguang Li, Zuoren Yang · 发表于：Plant Biotechnology Journal · 年份：2023 · DOI：10.1111/pbi.14005 · 被引用次数：15 · 研究领域：Research in Cotton Cultivation

Cotton is one of the most important natural fibre resources in the textile industry that has been cultivated to produce long and spinnable fibres. These cotton fibres are elongated single cells that are derived from the outer epidermis cells of ovules, which are an ideal model to study cell elongation in the plant kingdom (Lee et al., 2007). Phytohormones, such as brassinosteroid (BR), have also been shown to promote fibre elongation. Additionally, many genes that regulate fibre elongation have been functionally identified, especially transcription factors. Still, our understanding of fibre elongation remains limited (Huang et al., 2021; Yang et al., 2020). Previously, PACLOBUTRAZOL RESISTANCE 1 (GhPRE1) was shown to be a target gene for fibre evolution, since it encodes an atypical Helix–Loop–Helix (HLH) protein. In allotetraploid cotton, GhPRE1D is silenced due to a TATA-box deletion from its promoter, and the retained GhPRE1A is specifically expressed in fibre cells, which plays an important role in fibre elongation (Zhao et al., 2018). However, the regulatory mechanism of GhPRE1A on fibre elongation still remains elusive. To dissect the molecular mechanism of fibre development, we used GhPRE1A as bait to screen its interacting proteins in a cotton fibre Y2H library. Y2H, BiFC and Co-IP experiments verified that GhPRE1A interacted with ATBS1 INTERACTING FACTOR 3 (GhAIF3) (Figure 1a–c), which was an atypical HLH protein (Figure 1d). In ectopic overexpression experiment, we ...