A Fur-regulated type VI secretion system contributes to oxidative stress resistance and virulence in Yersinia pseudotuberculosis

作者：Yuxin Zuo, Changfu Li, Danyang Yu, Kenan Wang, Yuqi Liu, Zhiyan Wei, Yantao Yang, Yao Wang, Xihui Shen, Lingfang Zhu · 发表于：Stress Biology · 年份：2023 · DOI：10.1007/s44154-022-00081-y · 被引用次数：16 · 研究领域：Vibrio bacteria research studies、Yersinia bacterium, plague, ectoparasites research、Antibiotic Resistance in Bacteria

Abstract The type VI secretion system (T6SS) is a widespread protein secretion apparatus deployed by many Gram-negative bacterial species to interact with competitor bacteria, host organisms, and the environment. Yersinia pseudotuberculosis T6SS4 was recently reported to be involved in manganese acquisition; however, the underlying regulatory mechanism still remains unclear. In this study, we discovered that T6SS4 is regulated by ferric uptake regulator (Fur) in response to manganese ions (Mn 2+ ), and this negative regulation of Fur was proceeded by specifically recognizing the promoter region of T6SS4 in Y. pseudotuberculosis . Furthermore, T6SS4 is induced by low Mn 2+ and oxidative stress conditions via Fur, acting as a Mn 2+ -responsive transcriptional regulator to maintain intracellular manganese homeostasis, which plays important role in the transport of Mn 2+ for survival under oxidative stress. Our results provide evidence that T6SS4 can enhance the oxidative stress resistance and virulence for Y. pseudotuberculosis . This study provides new insights into the regulation of T6SS4 via the Mn 2+ -dependent transcriptional regulator Fur, and expands our knowledge of the regulatory mechanisms and functions of T6SS from Y. pseudotuberculosis .