Structural elucidation of a novel arabinogalactan LFP-80-W1 from Lycii fructus with potential immunostimulatory activity

作者：Xiaofei Liang, Mengqiu Liu, Sheng Guo, Fang Zhang, Wanchen Cui, Fei Zeng, Mingming Xu, Dawei Qian, Jin‐Ao Duan · 发表于：Frontiers in Nutrition · 年份：2023 · DOI：10.3389/fnut.2022.1067836 · 被引用次数：12 · 研究领域：Polysaccharides and Plant Cell Walls、Polysaccharides Composition and Applications、Seaweed-derived Bioactive Compounds

Polysaccharides are the most important effective components of Lycii fructus, which has a variety of biological activities and broad application prospects in the fields of medicine and food. In this study, we reported a novel arabinogalactan LFP-80-W1 with potential immunostimulatory activity. LFP-80-W1 was a continuous symmetrical single-peak with an average molecular weight of 4.58 × 10 4 Da and was mainly composed of arabinose and galactose. Oligosaccharide sequencing analyses and NMR data showed that the LFP-80-W1 domain consists of a repeated 1,6-linked β-Gal p main chain with branches arabinoglycan and arabinogalactan at position C-3. Importantly, we found that LFP-80-W1 could activate the MAPK pathway and promote the release of NO, IL-6, and TNF-α cytokines in vitro . Therefore, our findings suggest that the homogeneous arabinogalactan from Lycii fructus, can be used as a natural immunomodulator.