Effect of calcium ionophore (A23187) on embryo development and its safety in PGT cycles

作者：Junya Zhang, Guidong Yao, Tongwei Zhang, Jingyi Hu, Guang Yang, Jiahuan He, Qina He, Huiying Fan, Yucheng Bai, Yingpu Sun · 发表于：Frontiers in Endocrinology · 年份：2023 · DOI：10.3389/fendo.2022.979248 · 被引用次数：17 · 研究领域：Reproductive Biology and Fertility、Prenatal Screening and Diagnostics、Reproductive System and Pregnancy

Background Intracytoplasmic sperm injection (ICSI) has tremendous advantages for resolving the problem of male infertility. However, ICSI fertilization can fail in some patients because of various reasons, primarily because of the failure of oocyte activation. Oocytes have been activated using calcium ionophore (A23187) in previous clinical cases of ICSI fertilization failure. However, studies on the efficiency of calcium ionophore (A23187) activation, its effects on the developmental potential of embryos, and its effects on pregnancy outcomes after embryo transfer are relatively limited. Methods In this study, we investigated the safety and long-term efficacy of calcium ionophore (A23187) by analyzing its effects on fertilization, embryonic development, aneuploidy, and pregnancy outcomes in patients undergoing preimplantation genetic testing (PGT) cycles. Results Comparative analyses of the activation followed by PGT (A-PGT) and PGT groups revealed no significant differences between the oocyte cleavage rate and high-quality embryo rate (98.19% vs. 98.63% and 63.13% vs. 68.39%, respectively, p > 0.05). Although the blastocyst formation rate was significantly lower in the A-PGT group than that in the PGT group (52.22% vs. 59.90%, p < 0.05), no significant difference was observed in the blastocyst aneuploidy rates of the two groups (24.49% vs. 24.55%, p > 0.05). Furthermore, no significant differences were observed between the two groups in terms of the liv...