Effects of Continuous LPS Induction on Oxidative Stress and Liver Injury in Weaned Piglets

作者：Yunxiao Zhou, Xiaofen Hu, Shengwei Zhong, Wanting Yu, Jue Wang, Wenlu Zhu, Tingyu Yang, Guotong Zhao, Yijie Jiang, Yong Li · 发表于：Veterinary Sciences · 年份：2022 · DOI：10.3390/vetsci10010022 · 被引用次数：28 · 研究领域：Immune Response and Inflammation、Immune Cell Function and Interaction、NF-κB Signaling Pathways

lipopolysaccharide (LPS) was established to explore the effects of continuous low-dose LPS induction on the mechanism of liver injury. A total of forty-eight healthy 28-day-old weaned piglets (weight = 6.65 ± 1.19 kg) were randomly divided into two groups: the CON group and LPS group. During the experimental period of thirteen days, the LPS group was injected intraperitoneally with LPS (100 μg/kg) once per day, and the CON group was treated with the same volume of 0.9% NaCl solution. On the 1st, 5th, 9th, and 13th days, the serum and liver of the piglets were collected for the determination of serum biochemical indexes, an antioxidant capacity evaluation, and histopathological examinations. In addition, the mRNA expression levels of the TLR4 pathway and inflammatory cytokines were detected. The results showed that the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) in the serum increased after LPS induction. The activities of total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-Px) in the serum and liver homogenate of the LPS group were lower than those of the CON group, while the malondialdehyde (MDA) content in the serum and the activities of catalase (CAT) and superoxide dismutase (SOD) in the liver of the LPS group were higher than those in the CON group. At the same time, morphological impairment of the livers occurred, including hepatocyte caryolysis, hepatocyte vacuolization, karyopycnosis, an...