Resistance to white spot syndrome virus in the European shore crab is associated with suppressed virion trafficking and heightened immune responses

作者：Rebecca S. Millard, Lisa K. Bickley, Kelly S. Bateman, Bas Verbruggen, Audrey Farbos, Anke Lange, Karen Moore, Grant D. Stentiford, Charles R. Tyler, Ronny van Aerle, Eduarda M. Santos · 发表于：Frontiers in Immunology · 年份：2022 · DOI：10.3389/fimmu.2022.1057421 · 被引用次数：11 · 研究领域：Invertebrate Immune Response Mechanisms、Neurobiology and Insect Physiology Research、Aquaculture disease management and microbiota

Introduction All decapod crustaceans are considered potentially susceptible to White Spot Syndrome Virus (WSSV) infection, but the degree of White Spot Disease (WSD) susceptibility varies widely between species. The European shore crab Carcinus maenas can be infected with the virus for long periods of time without signs of disease. Given the high mortality rate of susceptible species, the differential susceptibility of these resistant hosts offers an opportunity to investigate mechanisms of disease resistance. Methods Here, the temporal transcriptional responses (mRNA and miRNA) of C. maenas following WSSV injection were analysed and compared to a previously published dataset for the highly WSSV susceptible Penaeus vannamei to identify key genes, processes and pathways contributing to increased WSD resistance. Results We show that, in contrast to P. vannamei , the transcriptional response during the first 2 days following WSSV injection in C. maenas is limited. During the later time points (7 days onwards), two groups of crabs were identified, a recalcitrant group where no replication of the virus occurred, and a group where significant viral replication occurred, with the transcriptional profiles of the latter group resembling those of WSSV-susceptible species. We identify key differences in the molecular responses of these groups to WSSV injection. Discussion We propose that increased WSD resistance in C. maenas may result from impaired WSSV endocytosis due to the inhibitio...