A circadian clock translational control mechanism targets specific mRNAs to cytoplasmic messenger ribonucleoprotein granules

作者：Kathrina Castillo, Cheng Wu, Zhaolan Ding, Osiris K. Lopez-Garcia, Emma Rowlinson, Matthew S. Sachs, Deborah Bell‐Pedersen · 发表于：Cell Reports · 年份：2022 · DOI：10.1016/j.celrep.2022.111879 · 被引用次数：20 · 研究领域：Light effects on plants、Photosynthetic Processes and Mechanisms、RNA and protein synthesis mechanisms

cells. About 14% of mRNAs are rhythmically translated in WT cells, and translation rhythms for ∼30% of these mRNAs, which we named circadian translation-initiation-controlled genes (cTICs), are dependent on the clock and CPC-3. Most cTICs are expressed from arrhythmic mRNAs and contain a P-body (PB) localization motif in their 5' leader sequence. Deletion of SNR-1, a component of cytoplasmic messenger ribonucleoprotein granules (cmRNPgs) that include PBs and stress granules (SGs), and the PB motif on one of the cTIC mRNAs, zip-1, significantly alters zip-1 rhythmic translation. These results reveal that the clock regulates rhythmic translation of specific mRNAs through rhythmic eIF2α activity and cmRNPg metabolism.