Analysis of gut microbiota in patients with acute myocardial infarction by 16S rRNA sequencing

作者：Xueyi Qian, Ankang Liu, Liang Chen, Lianjun He, Zhenyu Xu, Shengxing Tang · 发表于：Annals of Translational Medicine · 年份：2022 · DOI：10.21037/atm-22-5671 · 被引用次数：21 · 研究领域：Gut microbiota and health、Clostridium difficile and Clostridium perfringens research、Oral microbiology and periodontitis research

Background: An increasing number of studies have shown that gut microbiota are associated with human cardiovascular disease, but the characteristics of intestinal flora in patients with acute myocardial infarction (AMI) are still unclear. In this study, we aimed to investigate the difference of intestinal microflora between patients with AMI and healthy people, and to find the effect of percutaneous coronary intervention (PCI) on intestinal microflora. Methods: A total of 60 stool samples and 60 peripheral blood samples were collected from 20 previously diagnosed AMI patients and 20 healthy people serving as controls. Gut microbiota communities were analyzed via 16 ribosomal RNA-sequencing (16S rRNA). Gut microbiota-derived metabolites, trimethylamine N-oxide (TMAO) and short-chain fatty acids (SCFA), in the blood were detected using stable isotope dilution high-performance liquid chromatography with on line electrospray ionization tandem mass spectrometry (LC/MS/MS). Results: showed a downward trend in AMI patients. During a longitudinal study to compare the changes in bacteria before and after treatment, we found routine cardiac admission therapy 1 week after PCI surgery had no effect on the microbial community structure in patients. There were significantly higher levels of plasma TMAO in AMI patients' microbiota than that in the control group. Contrarily, there was no obvious change in SCFA. Conclusions: The gut microbiota of patients with AMI differs from that of normal ...