Hormetic Effect of Pyroligneous Acids on Conjugative Transfer of Plasmid-mediated Multi-antibiotic Resistance Genes within Bacterial Genus

作者：Mengying Shao, Liuqingqing Liu, Bingjie Liu, Hao Zheng, Meng Wei, Yifan Liu, Xiao Zhang, Xiaohan Ma, Cuizhu Sun, Xianxiang Luo, Fengmin Li, Baoshan Xing · 发表于：ACS Environmental Au · 年份：2022 · DOI：10.1021/acsenvironau.2c00056 · 被引用次数：29 · 研究领域：Pharmaceutical and Antibiotic Environmental Impacts、Antibiotic Resistance in Bacteria

High Resolution Image Download MS PowerPoint Slide Spread of antibiotic resistance genes (ARGs) by conjugation poses great challenges to public health. Application of pyroligneous acids (PA) as soil amendments has been evidenced as a practical strategy to remediate pollution of ARGs in soils. However, little is known about PA effects on horizontal gene transfer (HGT) of ARGs by conjugation. This study investigated the effects of a woody waste-derived PA prepared at 450°C and its three distillation components (F1, F2, and F3) at different temperatures (98, 130, and 220°C) on conjugative transfer of plasmid RP4 within Escherichia coli . PA at relatively high amount (40–100 μL) in a 30-mL mating system inhibited conjugation by 74–85%, following an order of PA > F3 ≈ F2 ≈ F1, proving the hypothesis that PA amendments may mitigate soil ARG pollution by inhibiting HGT. The bacteriostasis caused by antibacterial components of PA, including acids, phenols, and alcohols, as well as its acidity (pH 2.81) contributed to the inhibited conjugation. However, a relatively low amount (10–20 μL) of PA in the same mating system enhanced ARG transfer by 26–47%, following an order of PA > F3 ≈ F2 > F1. The opposite effect at low amount is mainly attributed to the increased intracellular reactive oxygen species production, enhanced cell membrane permeability, increased extracellular polymeric substance contents, and reduced cell surface charge. Our findings highlight the hormesis (low-amount prom...