Living Probiotics - Loaded Hydrogel Microspheres with Gastric Acid Resistance and ROS Triggered Release for Potential Therapy of Inflammatory Bowel Disease

作者：Yi Zhu, Qinghua Wang, Yu Chen, Yuntao Xie, Guohao Han, Shan Liu, Yeping Song, Weipeng Lu, Yanchuan Guo · 发表于：ACS Applied Polymer Materials · 年份：2022 · DOI：10.1021/acsapm.2c01893 · 被引用次数：32 · 研究领域：Advanced Drug Delivery Systems、Cystic Fibrosis Research Advances、Inhalation and Respiratory Drug Delivery

Probiotics have played a critical role in regulating intestinal flora balance and have been considered as an effective and safe strategy for treating inflammatory bowel disease (IBD). However, it is still a huge challenge to precisely deliver probiotics to the inflammatory area while maintaining their high activity. Herein, a well-designed strategy is provided to construct hydrogel microspheres (HMs) through microencapsulation for loading living probiotics, which realizes a high encapsulation efficiency of 95.24%. By chemically cross-linking with 1,4-phenylenediboronic acid (Pa), the microspheres present a sensitive reactive oxygen species (ROS) response for targeted delivery and controlled release of the probiotics. In vitro simulated experiments demonstrate the high viability of the encapsulated probiotics against the simulated gastric (>1 × 10 4 CFU/mL) and intestinal (>2 × 10 7 CFU/mL) fluid environments, which are higher than the data (0 and 1 × 10 7 CFU/mL) of the free cells, respectively. Moreover, the microspheres exhibited nontoxicity and favorable biodegradability, ensuring their safe use in vivo . This work provides a universal and viable alternative to precisely deliver highly active probiotics to intestinal inflammatory areas, promising to meet the needs of targeted IBD therapy.