Extensive diversity of RNA viruses in ticks revealed by metagenomics in northeastern China

作者：Ziyan Liu, Liang Li, Wenbo Xu, Yongxu Yuan, Xiaojie Liang, Li Zhang, Zhengkai Wei, Liyan Sui, Yinghua Zhao, Yanyan Cui, Qing Yin, Dajun Li, Qianxue Li, Zhijun Hou, Feng Wei, Quan Liu, Zedong Wang · 发表于：PLoS neglected tropical diseases · 年份：2022 · DOI：10.1371/journal.pntd.0011017 · 被引用次数：63 · 研究领域：Viral Infections and Vectors、Vector-Borne Animal Diseases、Viral Infections and Outbreaks Research

BACKGROUND: Ticks act as important vectors of infectious agents, and several emerging tick-borne viruses have recently been identified to be associated with human diseases in northeastern China. However, little is known about the tick virome in northeastern China. METHODS: Ticks collected from April 2020 to July 2021 were pooled for metagenomic analysis to investigate the virome diversity in northeastern China. RESULTS: In total, 22 RNA viruses were identified, including four each in the Nairoviridae and Phenuiviridae families, three each in the Flaviviridae, Rhabdoviridae, and Solemoviridae families, two in the Chuviridae family, and one each in the Partitiviridae, Tombusviridae families and an unclassified virus. Of these, eight viruses were of novel species, belonging to the Nairoviridae (Ji'an nairovirus and Yichun nairovirus), Phenuiviridae (Mudanjiang phlebovirus), Rhabdoviridae (Tahe rhabdovirus 1-3), Chuviridae (Yichun mivirus), and Tombusviridae (Yichun tombus-like virus) families, and five members were established human pathogens, including Alongshan virus, tick-borne encephalitis virus, Songling virus, Beiji nairovirus, and Nuomin virus. I. persulcatus ticks had significant higher number of viral species than H. japonica, H. concinna, and D. silvarum ticks. Significant differences in tick viromes were observed among Daxing'an, Xiaoxing'an and Changbai mountains. CONCLUSIONS: These findings showed an extensive diversity of RNA viruses in ticks in northeastern China,...