Root-Zone Restriction Regulates Soil Factors and Bacterial Community Assembly of Grapevine

作者：Muhammad Zahid, Muzammil Hussain, Yue Song, Jiajia Li, Dinghan Guo, Xiangyi Li, Shiren Song, Lei Wang, Wenping Xu, Shiping Wang · 发表于：International Journal of Molecular Sciences · 年份：2022 · DOI：10.3390/ijms232415628 · 被引用次数：10 · 研究领域：Mycorrhizal Fungi and Plant Interactions、Plant-Microbe Interactions and Immunity、Plant Pathogens and Fungal Diseases

Root-zone restriction induces physiological stress on roots, thus limiting the vegetative and enhancing reproductive development, which promotes fruit quality and growth. Numerous bacterial-related growth-promoting, stress-mitigating, and disease-prevention activities have been described, but none in root-restricted cultivation. The study aimed to understand the activities of grapevine bacterial communities and plant-bacterial relationships to improve fruit quality. We used High-throughput sequencing, edaphic soil factors, and network analysis to explore the impact of restricted cultivation on the diversity, composition and network structure of bacterial communities of rhizosphere soil, roots, leaves, flowers and berries. The bacterial richness, diversity, and networking were indeed regulated by root-zone restriction at all phenological stages, with a peak at the veraison stage, yielding superior fruit quality compared to control plants. Moreover, it also handled the nutrient availability in treated plants, such as available nitrogen (AN) was 3.5, 5.7 and 0.9 folds scarcer at full bloom, veraison and maturity stages, respectively, compared to control plants. Biochemical indicators of the berry have proved that high-quality berry is yielded in association with the bacteria. Cyanobacteria were most abundant in the phyllosphere, Proteobacteria in the rhizosphere, and Firmicutes and Bacteroidetes in the endosphere. These bacterial phyla were most correlated and influenced by diff...