Automated assembly scaffolding using RagTag elevates a new tomato system for high-throughput genome editing

作者：Michael Alonge, Ludivine Lebeigle, Melanie Kirsche, Katie Jenike, Shujun Ou, Sergey Aganezov, Xingang Wang, Zachary B. Lippman, Michael C. Schatz, Sebastian Soyk · 发表于：Genome biology · 年份：2022 · DOI：10.1186/s13059-022-02823-7 · 被引用次数：1022 · 研究领域：CRISPR and Genetic Engineering、Plant Virus Research Studies、Chromosomal and Genetic Variations

Advancing crop genomics requires efficient genetic systems enabled by high-quality personalized genome assemblies. Here, we introduce RagTag, a toolset for automating assembly scaffolding and patching, and we establish chromosome-scale reference genomes for the widely used tomato genotype M82 along with Sweet-100, a new rapid-cycling genotype that we developed to accelerate functional genomics and genome editing in tomato. This work outlines strategies to rapidly expand genetic systems and genomic resources in other plant species.