Nanoprobe Based on Biominerals in Protein Corona for Dual-Modality MR Imaging and Therapy of Tumors

作者：Peisen Zhang, Yuanyuan Qiao, Lichong Zhu, Meng Qin, Qilong Li, Chuang Liu, Yuping Xu, Xin Zhang, Zhihua Gan, Yi Hou · 发表于：ACS Nano · 年份：2022 · DOI：10.1021/acsnano.2c05917 · 被引用次数：67 · 研究领域：Nanoparticle-Based Drug Delivery、Nanoplatforms for cancer theranostics、Graphene and Nanomaterials Applications

Various functional nanomaterials have been fabricated as diagnostic and therapeutic nanomedicines; however, the nanoparticles closely interact with proteins when immersed in biological fluids, forming a “protein corona” that critically alters the biological identity of nanomedicine. Here, we developed a robust strategy to construct theranostic nanoprobes based on protein-corona-coated Fe 3 O 4 nanoparticles and biomineralization in the corona. Water-soluble carboxylic Fe 3 O 4 nanoparticles were prepared by treating oleate-capped Fe 3 O 4 nanoparticles with Lemieux–von Rudloff reagent. Bovine serum albumin (BSA) was used as a model protein to form a corona on the surface of Fe 3 O 4 nanoparticles, endowing the Fe 3 O 4 nanoparticles with biocompatibility and nonimmunogenicity. The protein corona also provides a template for biomimetic mineralization of Fe 3+ with tannic acid (TA) to construct Fe 3 O 4 @BSA-TAFe III nanoprobes. The TA-Fe(III) biominerals can not only act as photothermal therapy agents but also interact with unsaturated transferrin in plasma to form a “hybrid” corona, enabling the nanoprobes to target tumor cells through the mediation of transferrin receptors, which commonly overexpress on tumor cell membranes. Once taken in by tumor cells, the protonation of phenol hydroxyl groups in acidic lysosomes would lead to the release of Fe 3+, inducing tumor cell death through a ferroptosis/apoptosis hybrid pathway. In addition, the released Fe 3+ can boost the T 1 -w...