Effects of straw returning combined with earthworm addition on nitrification and ammonia oxidizers in paddy soil

作者：Xiangqian Chu, Naling Bai, Xianqing Zheng, Quanhua Wang, Xi Pan, Shuangxi Li, Juanqin Zhang, Haiyun Zhang, Haiyun Zhang, Wenjie He, Feng Zhong, Weiguang Lv, Hanlin Zhang, Hanlin Zhang · 发表于：Frontiers in Microbiology · 年份：2022 · DOI：10.3389/fmicb.2022.1069554 · 被引用次数：17 · 研究领域：Soil Carbon and Nitrogen Dynamics、Constructed Wetlands for Wastewater Treatment、Biocrusts and Microbial Ecology

Introduction: Soil ammonia oxidation, which acts as the first and rate-limiting step of nitrification, is driven by ammonia-oxidizing bacteria (AOB), ammonia-oxidizing archaea (AOA) and complete ammonia oxidizer (comammox, amoA gene of clade-A and clade-B). Straw returning, widely used ecological technology in China, is an effective measure for promoting straw decomposition and soil nutrient cycling when combined with earthworm addition. However, the effects of straw returning combined with earthworm addition on soil ammonia oxidizers remain poorly understood. Methods: A 2-year plot experiment was conducted with 5 treatments: no fertilizer (CK); regular fertilization (RT); straw returning (SR); earthworm addition (W); straw returning + earthworm addition (SRW). The AOA, AOB, comammox clade-A and clade-B community microbial diversities and structures were investigated by high-throughput sequencing. Results: < 0.05). Discussion: This study provides scientific support for the application of straw returning combined with earthworm addition to improve soil nitrification with respect to soil ammonia-oxidizing microorganisms.