Bioprinting of alginate-carboxymethyl chitosan scaffolds for enamel tissue engineering in vitro

作者：Fatemeh Mohabatpour, Xiaoman Duan, Zahra Yazdanpanah, Xavier Lee Tabil, Liubov Lobanova, Ning Zhu, Silvana Papagerakis, Daniel Chen, Pétros Papagerakis · 发表于：Biofabrication · 年份：2022 · DOI：10.1088/1758-5090/acab35 · 被引用次数：60 · 研究领域：3D Printing in Biomedical Research、Bone Tissue Engineering Materials、Additive Manufacturing and 3D Printing Technologies

Abstract Tissue engineering offers a great potential in regenerative dentistry and to this end, three dimensional (3D) bioprinting has been emerging nowadays to enable the incorporation of living cells into the biomaterials (such a mixture is referred as a bioink in the literature) to create scaffolds. However, the bioinks available for scaffold bioprinting are limited, particularly for dental tissue engineering, due to the complicated, yet compromised, printability, mechanical and biological properties simultaneously imposed on the bioinks. This paper presents our study on the development of a novel bioink from carboxymethyl chitosan (CMC) and alginate (Alg) for bioprinting scaffolds for enamel tissue regeneration. CMC was used due to its antibacterial ability and superior cell interaction properties, while Alg was added to enhance the printability and mechanical properties as well as to regulate the degradation rate. The bioinks with three mixture ratios of Alg and CMC (2–4, 3–3 and 4–2) were prepared, and then printed into the calcium chloride crosslinker solution (100 mM) to form a 3D structure of scaffolds. The printed scaffolds were characterized in terms of structural, swelling, degradation, and mechanical properties, followed by their in vitro characterization for enamel tissue regeneration. The results showed that the bioinks with higher concentrations of Alg were more viscous and needed higher pressure for printing; while the printed scaffolds were highly porous and...