Whole-genome analysis revealed the growth-promoting mechanism of endophytic bacterial strain Q2H1 in potato plants

作者：Yuhu Wang, Qianqian Zhao, Zhenqi Sun, Yahui Li, Hongtao He, Yuanyu Zhang, Xiangdong Yang, Dong Wang, Baozhu Dong, Hongyou Zhou, Mingmin Zhao, Hongli Zheng · 发表于：Frontiers in Microbiology · 年份：2022 · DOI：10.3389/fmicb.2022.1035901 · 被引用次数：39 · 研究领域：Plant-Microbe Interactions and Immunity、Plant Pathogenic Bacteria Studies、Microbial Natural Products and Biosynthesis

Introduction Endophytes are non-pathogenic inhabitants of healthy plant tissues and have been found to promote plant growth and health. The endophytic bacterial strain Q2H1 was isolated from the roots of the potato and was identified to exhibit growth-promoting effects in potato plants. Methods Whole-genome sequencing was performed to reveal the mechanism underlying its growth-promoting effect. The obtained sequencing data of approximately 5.65 MB encompassed 5,533 coding sequences. Of note, nine secondary metabolite gene clusters, including siderophore gene clusters, closely associated with plant growth promotion (PGP) were predicted by antiSMASH software. Comparative genomic analysis revealed that Q2H1 belongs to the genus Peribacillus . By gene function annotation, those genes related to plant growth-promoting activities, including indole-3-acetic acid (IAA) synthesis in tryptophan metabolism, siderophore biosynthetic activity, phosphate solubilization, nitrogen fixation, and related genes, were summarized. IAA (14.4 μg/ml) was presumptively produced by Q2H1 using the Salkowski colorimetric method. A total of five genes, namely, phoU, pstB, pstA1, pstC , and pstS , were annotated for phosphate solubilization, which is associated with the ability of the Q2H1 strain to solubilize phosphate under in vitro conditions. Results It is revealed that genes in the Q2H1 genome associated with nitrogen fixation belonged to three groups, namely, nitrogen fixation ( nifU, sufU, salA , a...