Zwitterionic Polysaccharide‐Based Hydrogel Dressing as a Stem Cell Carrier to Accelerate Burn Wound Healing

作者：Qingyu Yu, Hong Sun, Zhiwei Yue, Chaojie Yu, Lijie Jiang, Xiaoru Dong, Mengmeng Yao, Mingyue Shi, Lei Liang, Yizao Wan, Hong Zhang, Fanglian Yao, Junjie Li · 发表于：Advanced Healthcare Materials · 年份：2022 · DOI：10.1002/adhm.202202309 · 被引用次数：108 · 研究领域：Wound Healing and Treatments、Mesenchymal stem cell research、Electrospun Nanofibers in Biomedical Applications

Stem cell therapy integrated with hydrogels has shown promising potential in wound healing. However, the existing hydrogels usually cannot reach the desired therapeutic efficacy for burn wounds due to the inadaptability to wound shape and weak anti-infection ability. Moreover, it is difficult to improve the environment for the survival and function of stem cells under complicated wound microenvironments. In this study, an injectable and self-healing hydrogel (DSC), comprising sulfobetaine-derived dextran and carboxymethyl chitosan, is fabricated through a Schiff-base reaction. Meanwhile, the DSC hydrogel shows high nonfouling properties, including resistance to bacteria and nonspecific proteins; moreover, the prepared hydrogel can provide a biomimetic microenvironment for cell proliferation whilst maintaining the stemness of adipose-derived stem cells (ADSCs) regardless of complex microenvironments. In burnt murine animal models, the ADSCs-laden hydrogel can significantly accelerate wound healing rate and scarless skin tissue regeneration through multiple pathways. Specifically, the ADSCs-laden DSC hydrogel can avoid immune system recognition and activation and thus reduce the inflammatory response. Moreover, the ADSCs-laden DSC hydrogel can promote collagen deposition, angiogenesis, and enhance macrophage M2 polarization in the wound area. In summary, sulfobetaine-derived polysaccharide hydrogel can serve as a versatile platform for stem cell delivery to promote burn wound h...