Clinical isolates of Mycobacterium tuberculosis with different genotypes exhibit distinct host macrophage responses in vitro

作者：Liang Chen, Xiaomeng Li, Qiao Li, Xuxia Zhang, Weicong Ren, Cong Yao, Yu Pang, Yi Liu, Chuanyou Li, Shenjie Tang · 发表于：Journal of Medical Microbiology · 年份：2022 · DOI：10.1099/jmm.0.001604 · 被引用次数：5 · 研究领域：Tuberculosis Research and Epidemiology、Mycobacterium research and diagnosis、Diagnosis and treatment of tuberculosis

Introduction . Mycobacterium tuberculosis ( M.tb ), the causative agent of tuberculosis, can survive as an intracellular parasite after entering macrophages via phagocytosis. M.tb strains are genotypically distinct and engage in diverse pathogen–host interactions, with different host immune responses triggered by different M.tb strains. Importantly, differences in intracellular accumulation and triggering of host macrophage responses during early infection stages are key determinants that shape the final outcomes of host innate immune responses to different M.tb strains. Hypothesis/Gap Statement . Clinical M.tb strains with different genotypes elicit different host innate immune responses in vitro . Aim . This work aimed to compare host innate immune responses elicited by genotypically diverse, clinically derived M.tb strains in vitro . Methodology . RAW264.7 cells were infected with three lineage 2 and lineage 4 clinically derived M.tb strains and strain H37Rv. Strains were evaluated for differences in intracellular growth, induction of macrophage apoptosis, and induction of expression of proinflammatory cytokines and associated pattern recognition receptors. Results . Highly variable cytokine profiles were observed subsequent to RAW264.7 cell infection with the different strains. The Beijing genotype strain, a modern Beijing strain belonging to lineage 2, induced milder host proinflammatory responses and less apoptosis and exhibited greater intracellular growth as compared ...