p16 INK4a Plays Critical Role in Exacerbating Inflammaging in High Fat Diet Induced Skin

作者：Yan Liang, Tianya Gu, Peng Su, Yi Lin, JiaBao Liu, Xiaoyan Wang, Xin Huang, Xiaodong Zhang, Jun Zhu, Lin Zhao, Changyan Fan, Guangyan Wang, Xin Gu, Jinde Lin · 发表于：Oxidative Medicine and Cellular Longevity · 年份：2022 · DOI：10.1155/2022/3415528 · 被引用次数：13 · 研究领域：Skin Protection and Aging、Telomeres, Telomerase, and Senescence、Cancer-related Molecular Pathways

Background . Long term high fat diets (HFD) promote skin aging pathogenesis, but detailed mechanisms remain unclear especially for inflammaging, which has recently emerged as a pathway correlating aging and age‐related disease with inflammation. p16 INK4a (hereafter termed p16) inhibits the cell cycle, with p16 deletion significantly inhibiting inflammaging. We observed that HFD‐induced p16 overexpression in the skin. Therefore, we investigated if p16 exacerbated inflammaging in HFD‐induced skin and also if p16 deletion exerted protective effects against this process. Methods . Eight‐week‐old double knockout (KO) ApoE -/- p16 -/- mice and ApoE -/- littermates were fed HFD for 12 weeks and their skin phenotypes were analyzed. We measured skin fibrosis, senescence‐associated secretory phenotype (SASP) levels, and integrin‐inflammasome pathway activation using histopathological, RNA‐sequencing (RNA‐seq), bioinformatics analysis, and molecular techniques. Results . We found that HFD contributed to inflammaging in the skin by activating the NLRP3 inflammasome pathway, increasing inflammatory infiltration, and promoting apoptosis by balancing expression between proapoptotic and antiapoptotic molecules. p16 knockout, when compared with the ApoE -/- phenotype, inhibited skin fibrosis by ameliorating inflammatory infiltration and proinflammatory factor expression (Interleukin‐1 β (IL‐1 β ), Interleukin‐6 (IL‐6), and tumor necrosis factor‐ α (TNF‐ α )), and also alleviated inflammaging...