Host transcriptome and microbiome interactions in Holstein cattle under heat stress condition

作者：Bartosz Czech, Yachun Wang, Kai Wang, Hanpeng Luo, Lirong Hu, Joanna Szyda · 发表于：Frontiers in Microbiology · 年份：2022 · DOI：10.3389/fmicb.2022.998093 · 被引用次数：16 · 研究领域：Effects of Environmental Stressors on Livestock、Agriculture Sustainability and Environmental Impact、Genetic and phenotypic traits in livestock

Climate change affects animal physiology. In particular, rising ambient temperatures reduce animal vitality due to heat stress and this can be observed at various levels which included genome, transcriptome, and microbiome. In a previous study, microbiota highly associated with changes in cattle physiology, which included rectal temperature, drooling score and respiratory score, were identified under heat stress conditions. In the present study, genes differentially expressed between individuals were selected representing different additive genetic effects toward the heat stress response in cattle in their production condition. Moreover, a correlation network analysis was performed to identify interactions between the transcriptome and microbiome for 71 Chinese Holstein cows sequenced for mRNA from blood samples and for 16S rRNA genes from fecal samples. Bioinformatics analysis was performed comprising: i) clustering and classification of 16S rRNA sequence reads, ii) mapping cows' transcripts to the reference genome and their expression quantification, and iii) statistical analysis of both data types-including differential gene expression analysis and gene set enrichment analysis. A weighted co-expression network analysis was carried out to assess changes in the association between gene expression and microbiota abundance as well as to find hub genes/microbiota responsible for the regulation of gene expression under heat stress. Results showed 1,851 differentially expressed g...