Distinct common signatures of gut microbiota associated with damp-heat syndrome in patients with different chronic liver diseases

作者：Yuqing Pan, Jianchun Guo, Na Hu, Yunhao Xun, Binbin Zhang, Qin Feng, Si Chen, Xiaojing Li, Qiaohong Liu, Yiyang Hu, Yu Zhao · 发表于：Frontiers in Pharmacology · 年份：2022 · DOI：10.3389/fphar.2022.1027628 · 被引用次数：28 · 研究领域：Gut microbiota and health、Clostridium difficile and Clostridium perfringens research、Dermatology and Skin Diseases

Background: Chronic hepatitis B (CHB) and non-alcoholic fatty liver disease (NAFLD) are prevalent in China. According to traditional Chinese medicine (TCM) theory, damp-heat (DH) syndrome is common in chronic liver disease. However, the biological characteristics related to quantitative diagnosis remain to be determined. This study aimed to identify the consistent alterations in the gut microbiota associated with DH syndrome in patients with CHB or NAFLD. Methods: A total of 405 individuals were recruited, of which 146 were participants who met the consistent TCM diagnosis by three senior TCM physicians and were typical syndromes. All participants were required to provide fresh stool and serum samples. The gut microbiota was assessed by fecal 16S rRNA gene sequencing, and the serum metabolite profiles of participants were quantified by an ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) system. DH syndrome-related bacteria taxa were identified based on the 146 individuals with typical syndromes and validated in all 405 volunteers. Results: The results showed that CHB and NAFLD patients with typical TCM DH syndrome had consistently elevated serum total bile acid (TBA) levels. Significant alterations in microbial community were observed according to TCM syndromes identification. A total of 870 microbial operational taxonomic units and 21 serum metabolites showed the same variation trends in both the CHB and NAFLD DH syndrome groups. The f...