Prevalence, risk factors and first record of mitochondrial cox1 gene-based molecular characterization of Paramphistomum epiclitum from Pakistan

作者：Mughees Aizaz Alvi, Ayed Alshammari, Faizan Asghar, Rana Muhammad Athar Ali, Li Li, Muhammad Saqib, Muhammad Kasib Khan, Muhammad Imran, Warda Qamar, Hussam Askar, Naser Abdelsater, Bao‐Quan Fu, Hongbin Yan, Wan‐Zhong Jia · 发表于：Frontiers in Veterinary Science · 年份：2022 · DOI：10.3389/fvets.2022.1018854 · 被引用次数：4 · 研究领域：Helminth infection and control、Parasite Biology and Host Interactions、Parasites and Host Interactions

Parasitic infestations are one of the major threats to the livestock industry in Pakistan. These have a negative impact on the production of domesticated livestock species. Paramphistomes belong to the superfamily Paramphistomoidea and are involved in infecting ruminants all over the world. To date, there was no information on mitochondrial DNA-based molecular characterization of Paramphistomum epiclitum from Pakistan. To close this research gap, this study was designed to provide insights into the epidemiology of Paramphistomum species. Paramphistomum epiclitum isolates were recovered from the rumen of small ruminants slaughtered at an abattoir located in Faisalabad city and animal demographics were recorded. DNA was extracted and mitochondrial cox 1 was amplified and sequenced. Prevalence was calculated along with a 95% confidence interval in various groups. The chi-square test was applied to determine the association between different variables under investigation. A phylogenetic tree was constructed based on the Bayesian method. Population diversity indices were calculated using DnaSP 4.5 software. A total of 43 mutations were observed among 7 haplotypes. Negative values of Fu's Fs values, and Tajima's D indicated population expansion. Deworming, season, and grazing were the variables that significantly correlate ( p < 0.05) with the prevalence of P . epiclitum . The high prevalence of P. epiclitum demonstrates that more studies are indeed needed to further underst...