Intrauterine infusion of a pathogenic bacterial cocktail is associated with the development of clinical metritis in postpartum multiparous Holstein cows

作者：J.C.C. Silva, Lucas Carvalho Siqueira, Marjory Xavier Rodrigues, M. Zinicola, Patrícia Wolkmer, Brianna Pomeroy, R.C. Bicalho · 发表于：Journal of Dairy Science · 年份：2022 · DOI：10.3168/jds.2022-21954 · 被引用次数：22 · 研究领域：Reproductive Physiology in Livestock、Ruminant Nutrition and Digestive Physiology、Animal health and immunology

Metritis is a uterine disorder common in dairy cattle caused by bacterial infection, with greater incidence in the early postpartum period. The disease causes delayed uterine involution, with a fetid, watery, red-brown discharge, with animals presenting different clinical signs including fever, dullness, inappetence, decreased milk yield, and dehydration. We developed an in vivo model of clinical metritis in Holstein multiparous cows using a pathogenic cocktail of Escherichia coli , Fusobacterium necrophorum , and Trueperella pyogenes . A total of 36 multiparous cows were randomly allocated to 1 of 3 treatment groups of 12 animals each. Cows assigned to the control group received an intrauterine administration of sterile saline solution, those in the low-dose group received a bacterial inoculum containing 10 6 cfu of Escherichia coli , Trueperella pyogenes , and Fusobacterium necrophorum ; and those in the high-dose group received 10 9 cfu of these same organisms, all within 24 h of parturition. Clinical signs; milk yield; dry matter intake; serum concentration of acute phase proteins, metabolites, and cytokines; and bacterial counts in vaginal discharge were measured from parturition until 14 d in milk. Based on clinical signs, cows in the low-dose group had a greater incidence of metritis (83.3%) compared with the control (9%) or high-dose (25%) group. The low-dose group also had lesser dry matter intake compared with controls and an acute phase protein profile that typifie...