Naïve B cells with low differentiation improve the immune reconstitution of HIV-infected patients

作者：Jie Jia, Yu Zhao, Ji-Qun Yang, Danfeng Lu, Xiuling Zhang, Jun-Hong Mao, Kunhua Wang, Jian‐Hua Wang, Yi‐Qun Kuang · 发表于：iScience · 年份：2022 · DOI：10.1016/j.isci.2022.105559 · 被引用次数：14 · 研究领域：Cytomegalovirus and herpesvirus research、Immune Cell Function and Interaction、HIV Research and Treatment

Incomplete immune reconstitution happens in some HIV-infected patients who have achieved persistent viral suppression under antiretroviral therapy (ART). We performed single-cell RNA sequencing for peripheral blood mononuclear cells to analyze B cell receptor (BCR) repertoire and B cell subtypes in health controls (non-HIV-infected, HCs), HIV-infected immunological responders (IRs), and immunological nonresponders (INRs). We found that the dominant usage of IGHV gene segments of naïve B cells and memory B cells were IGHV3 and IGHV4 , and the diversity of BCR repertoire was decreased in INRs. Differentiation trajectory analysis showed that the low differentiation of naïve B cells was related to satisfactory immune status. The cell cycle of B cells with immune-specific genes of IgD + B cells was degraded in INRs, which was mediated by the anaphase-promoting complex/cyclosome pathway in the phase of G2/M checkpoints. These findings provide significant insights to understand the function of B cell-mediated immune response in immune reconstitution after HIV infection.