Soil properties rather than plant diversity mediate the response of soil bacterial community to N and P additions in an alpine meadow

作者：Zhenrong Lin, Lina Shi, Xiaoting Wei, Bing Han, Cuoji Peng, Zeying Yao, Qing Xiao, Xinmin Lu, Yanfang Deng, Huakun Zhou, Kesi Liu, Xinqing Shao · 发表于：Frontiers in Microbiology · 年份：2022 · DOI：10.3389/fmicb.2022.1036451 · 被引用次数：12 · 研究领域：Soil Carbon and Nitrogen Dynamics、Peatlands and Wetlands Ecology、Microbial Community Ecology and Physiology

The alpine meadow on the Qinghai-Tibetan Plateau, which is susceptible to global climate change and human activities, is subject to nutrient addition such as nitrogen (N) and phosphorus (P) to enhance soil available nutrients and ecosystem productivity. Soil bacterial community partly drivers the effects of nutrient additions on ecosystem processes, whereas the factors influencing N and P additions on bacterial community in alpine meadows are not well documented. We conducted a N and P addition experiment in an alpine meadow ecosystem on the Qinghai-Tibetan Plateau with four treatments: untreated control (CK), N addition (N), P addition (P), and NP addition (NP). We employed a high-throughput Illumina Miseq sequencing technology to investigate the response of soil bacterial community to short-term N and P additions. N and P additions decreased soil bacterial richness (OTU numbers and Chao 1 index), and P addition decreased soil bacterial diversity (Shannon and Simpson indices). N addition directly induced the change of soil N H 4 + − N , and decreased plant diversity. The N and P additions reduced soil bacterial community diversity, whose response was independent with plant diversity. Additionally, nutrient additions altered soil bacterial community composition, which were highly correlated with soil properties (i.e. pH, N H 4 + − N , and TP) as shown by RDA. Consistently, structural equation modeling results revealed that N addition indirectly acted...