Molecular characterization of three peroxiredoxin genes in Portunus pelagicus expressed in response to Vibrio alginolyticus challenge

作者：Ke Liu, Jinxia Liu, Zining Zhang, Tianjiao Ren, Min Lu, Meixuan Lei, Solomon Felix Dan, Zhenyu Lan, Zihang Ma, Huaiyi Fang, Yan Zhang, Peng Zhu, Yongyan Liao · 发表于：Aquaculture Reports · 年份：2022 · DOI：10.1016/j.aqrep.2022.101391 · 被引用次数：5 · 研究领域：Environmental Toxicology and Ecotoxicology、Redox biology and oxidative stress、Marine Biology and Environmental Chemistry

Portunus pelagicus inhabits coastal muddy or sandy seabed, and are often exposed to rotten sewage containing a large number of bacteria. In this study, RT-PCR technology was used to clone peroxiredoxin (prx) genes prx 1 , prx 3 and prx 6 of P. pelagicus. Genes expressed in normal tissue, gills and hepatopancreas after Vibrio alginolyticus stress were tested by RT-qPCR. The full-length coding sequences of prx 1 , prx 3 and prx 6 were 597 bp, 690 bp and 660 bp, which encode 198, 229 and 219 amino acids, respectively. Prx1 and prx 3 are typical 2-cys peroxiredoxins, while prx 6 is a typical 1-cys peroxiredoxin. RT-qPCR revealed widespread expression of prx 1 , prx 3 and prx 6 in all the tested tissues, and their expression was the highest in gills (93.81-fold vs muscle tissue), hepatopancreas (28.95-fold vs thoracic ganglia) and gonads (219.43-fold vs thoracic ganglia), respectively. The highest expression of prx 1 , prx 3 and prx 6 in the hepatopancreas of surviving individuals belonging to the experimental group were 1.67-, 1.86- and 2.11-fold higher than the control group after V. alginolyticus challenge, and the highest expression in gills was 5.99-, 5.51- and 6.11-fold higher than in the control group, respectively. However, the expression of prx genes in dead individuals of the experimental group was lower than the surviving individuals. These results revealed that prx genes play an important role in the defense against V. alginolyticus stress. The cause of death under sub...