First Report of Leaf Sheath Disease, Caused by Microdochium seminicola , on Zizania latifolia in China

作者：Mengfei Yang, Y. P. Li, Zhaisheng Zheng, Runxian Song, S. F. Zhang · 发表于：Plant Disease · 年份：2022 · DOI：10.1094/pdis-07-22-1701-pdn · 被引用次数：2 · 研究领域：Plant Pathogens and Fungal Diseases、Plant-Microbe Interactions and Immunity、Plant Disease Resistance and Genetics

Zizania latifolia, which belongs to the tribe Oryzea, has been widely grown over thousands of years as an aquatic vegetable in China. In the autumn of 2019, the disease harmful to the leaf sheaths of Z. latifolia was found in Jinhua City and Lishui City, Zhejiang Province. The disease caused brown oval-shaped spots (3~5 cm long, 0.5~1 cm wide) on leaf sheaths (Figure 1A). The infected plants produced non-commercial Jiaobai. The surface of the Jiaobai was pale yellow instead of white, and the length of the Jiaobai was 25% shorter than the uninfected plants. To 2022, the disease has been found in most planting areas of Z. latifolia in Zhejiang, Jiangsu, Fujian, Jiangxi and Anhui provinces in China. About 20%~50% incidence of the disease in these main production areas has seriously threatened the yield and quality of Jiaobai. To identify the causal agent, diseased leaf sheaths were collected. Symptomatic leaf sheath tissue was sterilized with 75% alcohol for 30 sec, and then washed three times with sterile distilled water for 1 min. A total of 37 strains were isolated on potato dextrose agar (PDA). We selected 10 strains for further morphological identification. An abundant mass of aerial mycelia was formed in 8 days (Figure 1B). The mycelium is transparent, septate and smooth. Conidia is fusiform, straight or curved, 0~3 septa, hyaline, tapering and rounding at the apex (Figure 1C and 1D). Strain XYQ3 was obtained through multi-generation culture. To further identify the pathog...