Exon-dependent transcriptional adaptation by exon-junction complex proteins Y14/RNP-4 and MAGOH/MAG-1 in Caenorhabditis elegans

作者：Jesús Fernandez-Abascal, Lei Wang, Bianca Graziano, Christina K. Johnson, Laura Bianchi · 发表于：PLoS Genetics · 年份：2022 · DOI：10.1371/journal.pgen.1010488 · 被引用次数：10 · 研究领域：Genetics, Aging, and Longevity in Model Organisms、RNA Research and Splicing、CRISPR and Genetic Engineering

Transcriptional adaptation is a powerful gene regulation mechanism that can increase genetic robustness. Transcriptional adaptation occurs when a gene is mutated and is mediated by the mutant RNA, rather than by protein feedback loops. We show here that transcriptional adaptation occurs in the C. elegans clh family of Cl- channels and that it requires exon-junction complex (EJC) proteins RNP-4, MAG-1, and eiF4AIII. Depending on which exons are deleted in distinct clh-1 alleles, different clh genes are regulated in an EJC-dependent manner. Our results support the idea that different transcriptional adaptation outcomes may be directed by the differential interaction of the EJC with its target mutant RNAs.