Identification of the major QTL QPm.cas-7D for adult plant resistance to wheat powdery mildew

作者：Hong Liu, Guohao Han, Tiantian Gu, Yuli Jin, Zhipeng Shi, Lixian Xing, Hanwen Yan, Jing Wang, Chenyang Hao, Meicheng Zhao, Diaoguo An · 发表于：Frontiers in Plant Science · 年份：2022 · DOI：10.3389/fpls.2022.1042399 · 被引用次数：7 · 研究领域：Wheat and Barley Genetics and Pathology、Plant Disease Resistance and Genetics、Plant pathogens and resistance mechanisms

Developing effective and durable host plant resistance is crucial for controlling powdery mildew, a devastating disease caused by Blumeria graminis f. sp. tritici ( Bgt ). In the present study, we dissected the genetic basis of the adult plant resistance to powdery mildew using a recombinant inbred line (RIL) composed of 176 F 9 RILs population derived from a cross between PuBing 3228 (P3228) and susceptible cultivar Gao 8901. P3228 exhibits stable adult-plant resistance to powdery mildew in the field over consecutive years. We identified two QTLs on chromosomes 7DS ( QPm.cas-7D ) and 1AL ( QPm.cas-1A ) contributed by P3228, and one QTL on 3DS ( QPm.cas-3D ) contributed by Gao 8901, which could explain 65.44%, 3.45%, and 2.18% of the phenotypic variances, respectively. By analyzing the annotated genes in the 1.168 Mb physical interval of the major QTL QPm.cas-7D , we locked a previously cloned adult-plant resistance gene Pm38 that was most probably the candidate gene of QPm.cas-7D. Sequence alignment analysis revealed that the candidate gene of QPm.cas-7D in P3228 was identical to the reported Pm38 sequence. Two haplotypes QPm-7D-R and QPm-7D-S were identified in the whole Pm38 genomic regions between P3228 and Gao 8901. To apply QPm.cas-7D in wheat breeding, we developed a kompetitive allele-specific PCR (KASP) marker Kasp5249 that is closely linked with these haplotypes. It is worth mentioning that the QPm-7D-R haplotype significantly decreased TKW and underwent negative se...