Effects of autochthonous strains mixture on gut microbiota and metabolic profile in cobia (Rachycentron canadum)

作者：Eric Amenyogbe, Jun Luo, Wei-jie Fu, Emmanuel Delwin Abarike, Zhongliang Wang, Jiansheng Huang, Christian Larbi Ayisi, Gang Chen · 发表于：Scientific Reports · 年份：2022 · DOI：10.1038/s41598-022-19663-x · 被引用次数：23 · 研究领域：Aquaculture disease management and microbiota、Aquaculture Nutrition and Growth、Aquatic life and conservation

Abstract The fish immune system is a topic or subject that offers a unique understanding of defensive system evolution in vertebrate heredity. While gut microbiota plays several roles in fish: well-being, promoting health and growth, resistance to bacterial invasion, regulation of energy absorption, and lipid metabolism. However, studies on fish gut microbiota face practical challenges due to the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits. This study was carried out to evaluate the impacts of supplemented three autochthonous strains, Bacillus sp. RCS1, Pantoea agglomerans RCS2, and Bacillus cereus RCS3 mixture diet on cobia fish ( Rachycentron canadum ). Also, chromatography, mass spectrometry and high throughput sequencing were combined to explore composition and metabolite profile of gut microbiota in juvenile cobia fed with supplemented diet. In the trial group, juvenile cobia received diets supplemented with 1 × 10 12 CFU mL −1 autochthonous strains for ten weeks and a control diet without supplementation. Juvenile cobia receiving diets supplementation exhibited significantly improved growth than those without additives (control). Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group. Similarly, digestive enzymes (trypsin, lipase, amylase, pepsin and c...