Characterization and genomic analysis of a nitrate reducing bacterium from shale oil in the Ordos Basin and the associated biosurfactant production

作者：Yuanyuan Xu, Fengdan Wei, Rui Xu, Tao Cheng, Yanling Ma · 发表于：Journal of environmental chemical engineering · 年份：2022 · DOI：10.1016/j.jece.2022.108776 · 被引用次数：9 · 研究领域：Microbial bioremediation and biosurfactants、Hydrocarbon exploration and reservoir analysis、Methane Hydrates and Related Phenomena

Bacterial metabolism plays an important role on the formation and exploitation of oil shale, and the cost-efficient and environmentally friendly disposal are of great significance for shale oil sustainable development. In the present study, a promising nitrate reducing bacterium was isolated from shale oil in the Ordos Basin with a superior ability to degrade long-chain alkane and produce biosurfactant, which was identified as Stenotrophomonas maltophilia designated strain WGB211 based on morphological and biochemical characteristics as well as 16 S rRNA sequence analysis. The WGB211 degraded a wide range of medium-long length n -alkanes and exhibited highly efficient in 32 C’s n -alkane degradation of over 73% removal rate within 7 days at 31 °C, pH 6.4 and 230 mg/L 32 C’s n -alkane under response surface methodology (RSM) predicted conditions. The biosurfactant product was identified as a mixture of lipopeptides and glycolipids through Fourier transform infrared spectroscopy (FT-IR). The whole genomic analysis showed that strain WGB211 contained a chromosome of 4913676 bp and 4472 protein-coding genes by Illumina NovaSeq. A great number of genes and enzymes related to alkane degradation, such as flavin-binding protein AlmA 1 and AlmA 2 , and long-chain alkane hydroxylase LadA, were identified based on gene annotation. RT-qPCR analysis showed that a dissimilarity of gene expression could be induced by 32 C's n -alkane. This study suggests that S. maltophilia WGB211 could be ...